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shuttle plasmid (pSET1 or pVA838) assemble containing the functional copy on the mutated gene fused in-frame to a cloned S. suisTo measure the speed of 89K excision, we produced a true-time quantitative PCR (qPCR) assay and identified that the frequency of 89K excision from an 05ZYH33 right away lifestyle was ∼0.032%, a fee comparable to that obs